RNA modifications detection by comparative Nanopore direct RNA sequencing

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dc.contributor.authorLeger, Adrien
dc.contributor.authorPAULO DE PAIVA ROSA AMARAL
dc.contributor.authorPandolfini, Luca
dc.contributor.authorCapitanchik, Charlotte
dc.contributor.authorCapraro, Federica
dc.contributor.authorMiano, Valentina
dc.contributor.authorMigliori, Valentina
dc.contributor.authorToolan-Kerr, Patrick
dc.contributor.authorSideri, Theodora
dc.contributor.authorEnright, Anton J.
dc.contributor.authorTzelepis, Konstantinos
dc.contributor.authorWerven, Folkert J. van
dc.contributor.authorLuscombe, Nicholas M.
dc.contributor.authorBarbieri, Isaia
dc.contributor.authorUle, Jernej
dc.contributor.authorFitzgerald, Tomas
dc.contributor.authorBirney, Ewan
dc.contributor.authorLeonardi, Tommaso
dc.contributor.authorKouzarides, Tony
dc.coverage.paisNão Informadopt_BR
dc.creatorLeger, Adrien
dc.creatorPandolfini, Luca
dc.creatorCapitanchik, Charlotte
dc.creatorCapraro, Federica
dc.creatorMiano, Valentina
dc.creatorMigliori, Valentina
dc.creatorToolan-Kerr, Patrick
dc.creatorSideri, Theodora
dc.creatorEnright, Anton J.
dc.creatorTzelepis, Konstantinos
dc.creatorWerven, Folkert J. van
dc.creatorLuscombe, Nicholas M.
dc.creatorBarbieri, Isaia
dc.creatorUle, Jernej
dc.creatorFitzgerald, Tomas
dc.creatorBirney, Ewan
dc.creatorLeonardi, Tommaso
dc.creatorKouzarides, Tony
dc.date.accessioned2022-12-10T18:20:02Z
dc.date.available2022-12-10T18:20:02Z
dc.date.issued2021
dc.description.notesTexto completopt_BR
dc.description.otherRNA molecules undergo a vast array of chemical post-transcriptional modifications (PTMs) that can affect their structure and interaction properties. In recent years, a growing number of PTMs have been successfully mapped to the transcriptome using experimental approaches relying on high-throughput sequencing. Oxford Nanopore direct-RNA sequencing has been shown to be sensitive to RNA modifications. We developed and validated Nanocompore, a robust analytical framework that identifies modifications from these data. Our strategy compares an RNA sample of interest against a non-modified control sample, not requiring a training set and allowing the use of replicates. We show that Nanocompore can detect different RNA modifications with position accuracy in vitro, and we apply it to profile m6A in vivo in yeast and human RNAs, as well as in targeted non-coding RNAs. We confirm our results with orthogonal methods and provide novel insights on the co-occurrence of multiple modified residues on individual RNA molecules.pt_BR
dc.format.extent17 p.pt_BR
dc.format.mediumDigitalpt_BR
dc.identifier.doi10.1038/s41467-021-27393-3pt_BR
dc.identifier.issue7198pt_BR
dc.identifier.urihttps://repositorio.insper.edu.br/handle/11224/4884
dc.identifier.volume12pt_BR
dc.language.isoInglêspt_BR
dc.publisherNão informadopt_BR
dc.relation.ispartofNature Communicationspt_BR
dc.rights.licenseO INSPER E ESTE REPOSITÓRIO NÃO DETÊM OS DIREITOS DE USO E REPRODUÇÃO DOS CONTEÚDOS AQUI REGISTRADOS. É RESPONSABILIDADE DOS USUÁRIOS INDIVIDUAIS VERIFICAR OS USOS PERMITIDOS NA FONTE ORIGINAL, RESPEITANDO-SE OS DIREITOS DE AUTOR OU EDITORpt_BR
dc.subject.keywordsNão informadopt_BR
dc.titleRNA modifications detection by comparative Nanopore direct RNA sequencingpt_BR
dc.typejournal article
dspace.entity.typePublication
local.identifier.sourceUrihttps://doi.org/10.1038/s41467-021-27393-3
local.subject.cnpqCiências Biológicaspt_BR
local.typeArtigo Científicopt_BR
relation.isAuthorOfPublication90769ac2-5975-4a9d-8316-ee9653a944bd
relation.isAuthorOfPublication.latestForDiscovery90769ac2-5975-4a9d-8316-ee9653a944bd

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